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Methanothermobacter/Methanobacterium Transduction Medium (DSMZ Medium 863)
[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]
Methanothermobacter/Methanobacterium
Transduction Medium
(DSMZ Medium 863)
Sodium carbonate solution ......................................................24.0mL
Trace elements solution...........................................................10.0mL
Cysteine solution .......................................................................5.0mL
NaS solution...............................................................................5.0mL
pH 7.0 ± 0.2 at 25°C
Sodium Carbonate Solution:
Composition per 100.0mL:
Na 2 CO 3 .......................................................................................10.6g
Preparation of Sodium Carbonate Solution: Add Na 2 CO 3 to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 25°C. Store anaerobically.
Basal Salts Solution:
KH 2 PO 4 .......................................................................................68.0g
NH 4 Cl .........................................................................................21.2g
Resazurin .................................................................................10.0mg
Preparation of Basal Salts Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Sparge with 100% N 2 .
Trace Elements Solution:
Nitrilotriacetic acid.....................................................................9.55g
MgCl 2 ·6H 2 O ...............................................................................4.06g
FeCl 2 ·4H 2 O.................................................................................0.98g
NaWO 4 ·6H 2 O ...........................................................................0.264g
NiCl 2 ·6H 2 O............................................................................... 0.118g
Na 2 Se 2 O 3 ......................................................................................0.1g
Na 2 MoO 4 ·4H 2 O........................................................................0.024g
CoCl 2 ·6H 2 O..............................................................................0.023g
Preparation of Trace Elements Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 7.0 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly.
Cysteine Solution:
Composition per 10.0mL:
L -Cysteine·HCl·H 2 O .....................................................................1.0g
Preparation of Cysteine Solution: Add L -cysteine·HCl·H 2 O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Adjust pH to 10.0. Sparge with 100% N 2 . Store anaerobically.
NaS Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................1.0g
Preparation of NaS Solution: Add Na 2 S·9H 2 O to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Adjust pH to
10.0. Sparge with N 2 . Store anaerobically.
Preparation of Medium: Prepare and dispense medium under 80%
N 2 + 20% CO 2 gas atmosphere. Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Anaerobically dis-
tribute 10.0mL aliquots into serum vials under 80% N 2 + 20% CO 2 gas
atmosphere. Seal vials. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to room temperature. Pressurize to 2 bar with 80% N 2 + 20% CO 2
gas atmosphere.
Use: For the transduction of Methanobacter spp. and Methanobacterium
spp.
Transduction Medium
(DSMZ Medium 863)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Basal salts solution ................................................................100.0mLSodium carbonate solution ......................................................24.0mL
Trace elements solution...........................................................10.0mL
Cysteine solution .......................................................................5.0mL
NaS solution...............................................................................5.0mL
pH 7.0 ± 0.2 at 25°C
Sodium Carbonate Solution:
Composition per 100.0mL:
Na 2 CO 3 .......................................................................................10.6g
Preparation of Sodium Carbonate Solution: Add Na 2 CO 3 to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 25°C. Store anaerobically.
Basal Salts Solution:
KH 2 PO 4 .......................................................................................68.0g
NH 4 Cl .........................................................................................21.2g
Resazurin .................................................................................10.0mg
Preparation of Basal Salts Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Sparge with 100% N 2 .
Trace Elements Solution:
Nitrilotriacetic acid.....................................................................9.55g
MgCl 2 ·6H 2 O ...............................................................................4.06g
FeCl 2 ·4H 2 O.................................................................................0.98g
NaWO 4 ·6H 2 O ...........................................................................0.264g
NiCl 2 ·6H 2 O............................................................................... 0.118g
Na 2 Se 2 O 3 ......................................................................................0.1g
Na 2 MoO 4 ·4H 2 O........................................................................0.024g
CoCl 2 ·6H 2 O..............................................................................0.023g
Preparation of Trace Elements Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 7.0 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly.
Cysteine Solution:
Composition per 10.0mL:
L -Cysteine·HCl·H 2 O .....................................................................1.0g
Preparation of Cysteine Solution: Add L -cysteine·HCl·H 2 O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Adjust pH to 10.0. Sparge with 100% N 2 . Store anaerobically.
NaS Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................1.0g
Preparation of NaS Solution: Add Na 2 S·9H 2 O to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Adjust pH to
10.0. Sparge with N 2 . Store anaerobically.
Preparation of Medium: Prepare and dispense medium under 80%
N 2 + 20% CO 2 gas atmosphere. Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Anaerobically dis-
tribute 10.0mL aliquots into serum vials under 80% N 2 + 20% CO 2 gas
atmosphere. Seal vials. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to room temperature. Pressurize to 2 bar with 80% N 2 + 20% CO 2
gas atmosphere.
Use: For the transduction of Methanobacter spp. and Methanobacterium
spp.
