Methanosarcina mazei Alpha Basal Medium

[所属分类:培养基配方] [发布时间:2021-5-26] [发布人:网站管理员2] [阅读次数:] [返回]

Methanosarcina mazei Alpha Basal Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaHCO 3 ........................................................................................4.4g
Pancreatic digest of casein............................................................2.0g
Yeast extract..................................................................................2.0g
NH 4 Cl ...........................................................................................1.0g
Na 2 S·6H 2 O....................................................................................0.5g
K 2 HPO 4 .........................................................................................0.4g
Sodium acetate·3H 2 O..................................................................0.27g
MgCl 2 ·6H 2 O................................................................................0.08g
CaCl 2 ·2H 2 O.................................................................................0.04g
CoCl 2 ·6H 2 O ...............................................................................1.5mg
FeSO 4 ·7H 2 O...............................................................................1.0mg
MnCl 2 ·4H 2 O...............................................................................1.0mg
Resazurin ...................................................................................1.0mg
H 3 BO 4 ........................................................................................0.1mg
NaMoO 4 ·2H 2 O...........................................................................0.1mg
ZnCl 2 ..........................................................................................0.1mg
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Prepare and dispense medium under 70%
N 2 + 30% CO 2 . Add components to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Sparge with 70%
N 2 + 30% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C. Asep-
tically and anaerobically distribute into sterile tubes or bottles.
Use:  For the cultivation of Methanosarcina mazei.