Methanosarcina acetivorans Medium

[所属分类:培养基配方] [发布时间:2021-5-26] [发布人:网站管理员2] [阅读次数:] [返回]

Methanosarcina acetivorans Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

NaCl............................................................................................23.4g
MgSO 4 ·7H 2 O..............................................................................9.45g
Na 2 CO 3 .........................................................................................5.0g
Yeast extract..................................................................................1.0g
NH 4 Cl ...........................................................................................1.0g
CaCl 2 ·2H 2 O ................................................................................0.14g
KCl................................................................................................0.1g
Na 2 HPO 4 .......................................................................................0.6g
L -Cysteine·HCl·H 2 O ................................................................0.025g
Na 2 S·9H 2 O ...............................................................................0.025g
Resazurin...................................................................................1.0mg
Trace elements solution...........................................................10.0mL
Methanol....................................................................................5.0mL
pH 6.8 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
Nitrilotriacetic acid.....................................................................12.8g
FeCl 3 ·6H 2 O.................................................................................1.35g
NaCl..............................................................................................1.0g
NiCl 2 ·6H 2 O.................................................................................0.12g
MnCl 2 ·4H 2 O .................................................................................0.1g
CaCl 2 ·2H 2 O ..................................................................................0.1g
ZnCl 2 .............................................................................................0.1g
Na 2 SeO 3 ·5H 2 O..........................................................................0.026g
CuCl 2 ·2H 2 O..............................................................................0.025g
CoCl 2 ·6H 2 O..............................................................................0.024g
Na 2 MoO 4 ·2H 2 O........................................................................0.024g
H 3 BO 3 .........................................................................................0.01g
Preparation of Trace Elements Solution:  Add nitrilotriacetic
acid to approximately 500.0mL of distilled/deionized water. Dissolve
by adding KOH and adjust pH to 6.5. Add remaining components.
Bring volume to 1.0L with additional distilled/deionized water. Adjust
pH to 7.0 with KOH.
Preparation of Medium: Prepare and dispense medium under 80%
N 2 + 20% CO 2 . Add components to distilled/deionized water and bring
volume to 1010.0mL. Mix thoroughly. Adjust pH to 7.0 with 1N HCl.
Sparge under 80% N 2 + 20% CO 2 for 3–4 min. Autoclave for 15 min
at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Methanosarcina acetivorans.