Methanol-Utilizing Bacteria Medium B

[所属分类:培养基配方] [发布时间:2021-5-25] [发布人:网站管理员2] [阅读次数:] [返回]

Methanol-Utilizing Bacteria Medium B

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 .......................................................................................3.0g
(NH 4 ) 2 SO 4 ....................................................................................3.0g
KH 2 PO 4 .........................................................................................1.4g
Yeast extract..................................................................................0.2g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O .............................................................................30.0mg
Ferric citrate.............................................................................30.0mg
MnCl 2 ·4H 2 O ..............................................................................5.0mg
ZnSO 4 ·7H 2 O..............................................................................5.0mg
CuSO 4 ·5H 2 O..............................................................................0.5mg
Methanol..................................................................................10.0mL
pH 7.1 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.1.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of methanol-utilizing bacteria.