Aeromonas Isolation Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Aeromonas Isolation Medium
Composition per liter:
Agar............................................................................................12.5g
Na 2 S 2 O 3 ....................................................................................10.67g
Special peptone.............................................................................5.0g
NaCl..............................................................................................5.0g
Xylose.........................................................................................3.75g
L- Lysine·HCl.................................................................................3.5g
Yeast extract..................................................................................3.0g
Sorbitol .........................................................................................3.0g
Bile salts........................................................................................3.0g
Inositol..........................................................................................2.5g
L- Arginine·HCl..............................................................................2.0g
Lactose..........................................................................................1.5g
Ferric ammonium citrate...............................................................0.8g
Bromthymol Blue.......................................................................0.04g
Thymol Blue...............................................................................0.04g
Ampicillin solution....................................................................2.5mL
pH 8.0 ± 0.1 at 25°C
Source: This medium without ampicillin is available from Sigma Al-
drich.
Ampicillin Solution:
Composition per 5.0mL:
Ampicillin................................................................................10.0mg
Preparation of Ampicillin Solution: Add ampicillin to distilled/
deionized water and bring volume to 5.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except ampicillin solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Do not autoclave. Cool to
50°C. Aseptically add 2.5mL of ampicillin solution. Pour into sterile
Petri dishes.
Use: For the isolation and selective differentiation of Aeromonas
hydrophila and other Aeromonas species from clinical specimens and
foods. Aeromonas species appear as small (0.5–1.5mm), dark green
colonies with darker centers.