Methanol Ammonium Salts Medium

[所属分类:培养基配方] [发布时间:2021-5-25] [发布人:网站管理员2] [阅读次数:] [返回]

Methanol Ammonium Salts Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

MgSO 4 ·7H 2 O................................................................................1.0g
NH 4 Cl ...........................................................................................0.5g
Na 2 HPO 4 .....................................................................................0.33g
KH 2 PO 4 .......................................................................................0.26g
CaCl 2 .............................................................................................0.2g
Ferrous EDTA............................................................................5.0mg
Na 2 MoO 4 ·2H 2 O.........................................................................2.0mg
FeSO 4 ·7H 2 O............................................................................500.0μg
ZnSO 4 ·7H 2 O...........................................................................400.0μg
EDTA......................................................................................250.0μg
CoCl 2 ·6H 2 O..............................................................................50.0μg
MnCl 2 ·4H 2 O .............................................................................20.0μg
H 3 BO 4 .......................................................................................15.0μg
NiCl 2 ·6H 2 O...............................................................................10.0μg
Methanol....................................................................................5.0mL
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add Na 2 HPO 4 and KH 2 PO 4 to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. In a
separate container, add remaining components, except methanol, to
distilled/deionized water and bring volume to 895.0mL. Mix thorough-
ly. Autoclave both solutions for 15 min at 15 psi pressure–121°C. Cool
to room temperature. Filter sterilize methanol. Aseptically add the ster-
ile phosphate solution and the sterile methanol to the cooled, sterile
basal medium. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use:  For the maintenance and cultivation of Methylomonas methy-
lotrophus.