Methanohalophilus mahii Medium

[所属分类:培养基配方] [发布时间:2021-5-25] [发布人:网站管理员2] [阅读次数:] [返回]

Methanohalophilus mahii Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

NaCl............................................................................................87.0g
MgCl 2 ·6H 2 O .................................................................................6.0g
NaHCO 3 ........................................................................................4.0g
Trypticase™..................................................................................2.0g
Yeast extract..................................................................................2.0g
KCl................................................................................................1.5g
NH 4 Cl ...........................................................................................1.0g
CaCl 2 ·2H 2 O ..................................................................................0.4g
K 2 HPO 4 ·3H 2 O ..............................................................................0.4g
Coenzyme M (mercaptoethane sulfonic acid)..............................0.2g
Resazurin ...................................................................................1.0mg
Trace elements solution...........................................................10.0mL
Na 2 S·9H 2 O solution.................................................................10.0mL
Methanol....................................................................................4.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
MgSO 4 ·7H 2 O............................................................................... 3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O............................................................................... 0.5g
FeSO 4 ·7H 2 O..................................................................................0.1g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CaCl 2 ·2H 2 O...................................................................................0.1g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
CuSO 4 ·5H 2 O...............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.01g
NiCl 2 ·6H 2 O...............................................................................0.025g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with
KOH. Add remaining components. Adjust pH to 7.0. Add distilled/de-
ionized water to 1.0L.
Na 2 S·9H 2 O Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O..................................................................................0.25g
Preparation of Na 2 S·9H 2 O Solution:  Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Prepare and dispense medium under 80%
N 2 + 20% CO 2 . Add components, except Na 2 S·9H 2 O solution, to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with
80% N 2 + 20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Aseptically and anaerobically add 10.0mL of sterile Na 2 S·9H 2 O solution.
Mix thoroughly.
Use:  For the cultivation and maintenance of Methanohalophilus
mahii.