Methanogenium Medium

[所属分类:培养基配方] [发布时间:2021-5-25] [发布人:网站管理员2] [阅读次数:] [返回]

Methanogenium Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................18.0g
NaHCO 3 ........................................................................................5.0g
MgSO 4 ·7H 2 O..............................................................................3.45g
MgCl 2 ·7H 2 O ...............................................................................2.75g
Yeast extract..................................................................................2.0g
Pancreatic digest of casein............................................................2.0g
Sodium acetate..............................................................................1.0g
L- Cysteine·HCl·H 2 O .....................................................................0.5g
Na 2 S·9H 2 O....................................................................................0.5g
KCl............................................................................................0.335g
NH 4 Cl .........................................................................................0.25g
CaCl 2 ·2H 2 O ................................................................................0.14g
K 2 HPO 4 .......................................................................................0.14g
Fe(NH 4 ) 2 (SO 4 ) 2 ·7H 2 O...............................................................2.0mg
Resazurin...................................................................................1.0mg
Trace elements solution SL-6..................................................10.0mL
Wolfe’s vitamin solution..........................................................10.0mL
pH 6.8 ± 0.2 at 25°C
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
Na 2 MoO 4 ·H 2 O ............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 · 2H 2 O.................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Wolfe’s Vitamin Solution:
Composition per liter:
Pyridoxine·HCl........................................................................10.0mg
Thiamine·HCl ............................................................................5.0mg
Riboflavin ..................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
Calcium pantothenate ................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Thioctic acid ..............................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Cyanocobalamin .....................................................................100.0μg
Preparation of Wolfe’s Vitamin Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  Prepare and dispense medium under 80%
N 2 + 20% CO 2 . Add components, except Wolfe’s vitamin solution, to
distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Adjust pH to 6.8. Autoclave for 15 min at 15 psi pressure–121°C.
Cool under 80% N 2 + 20% CO 2 . Aseptically add sterile Wolfe’s vita-
min solution. Aseptically and anaerobically distribute into sterile tubes
or flasks.
Use:  For the cultivation and maintenance of Methanococcus frisius,
Methanococcus maripaludis, Methanococcus thermolithotrophicus,
and Methanoplanus limicola.