Methanogen Medium, Zeikus

[所属分类:培养基配方] [发布时间:2021-5-25] [发布人:网站管理员2] [阅读次数:] [返回]

Methanogen Medium, Zeikus

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Inorganic salts solution..........................................................500.0mL
Vitamin solution.....................................................................500.0mL
Na 2 S·9H 2 O solution.................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Inorganic Salts Solution:
Composition per 500.0mL:
K 2 HPO 4 ·3H 2 O ............................................................................1.45g
NH 4 Cl ...........................................................................................1.0g
KH 2 PO 4 .......................................................................................0.75g
MgCl 2 ·6H 2 O .................................................................................0.2g
Nitrilotriacetic acid.....................................................................0.04g
CaCl 2 ·2H 2 O ................................................................................0.02g
FeCl 2 ·4H 2 O................................................................................3.6mg
CoCl 2 ·6H 2 O...............................................................................1.5mg
MnCl 2 ·4H2O..............................................................................0.9mg
ZnCl 2 ..........................................................................................0.9mg
H 3 BO 2 ......................................................................................0.17mg
Na 2 MoO 4 ·2H 2 O..........................................................................09mg
Preparation of Inorganic Salts Solution: Add nitrilotriacetic
acid to 250.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Readjust pH to 7.2 with
H 2 SO 4 or KOH. Add distilled/deionized water to 500.0mL. Filter ster-
ilize.
Vitamin Solution:
Composition per 500.0mL:
Pyridoxine·HCl..........................................................................1.0mg
p-Aminobenzoic acid.................................................................0.5mg
Ca- D- pantothenate......................................................................0.5mg
Nicotinic acid.............................................................................0.5mg
Riboflavin..................................................................................0.5mg
Thiamine·HCl............................................................................0.5mg
Thioctic acid..............................................................................0.5mg
Biotin .........................................................................................0.2mg
Folic acid....................................................................................0.2mg
Vitamin B 12 ..............................................................................0.01mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 500.0mL. Mix thoroughly. Filter
sterilize.
Na 2 S·9H 2 O Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................0.3g
Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Prepare and distribute medium anaerobi-
cally under 95% N 2 + 5% CO 2 . Aseptically and anaerobically combine
500.0mL of sterile inorganic salts solution, 500.0mL of sterile vitamin
solution, and 10.0mL of sterile Na 2 S·9H 2 O solution. Mix thoroughly.
Aseptically and anaerobically distribute into sterile tubes or flasks.
Use: For the cultivation of methanogenic bacteria.