Methanogen Enrichment Medium, Barker

[所属分类:培养基配方] [发布时间:2021-5-25] [发布人:网站管理员2] [阅读次数:] [返回]

Methanogen Enrichment Medium, Barker

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

CaCO 3 .........................................................................................20.0g
NH 4 Cl ...........................................................................................1.0g
K 2 HPO 4 ·3H 2 O...............................................................................0.4g
MgCl 2 ·6H 2 O..................................................................................0.1g
Methanol..................................................................................20.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except methanol and
CaCO 3 , to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 25°C. Aseptically add filter-sterilized
methanol solution. Mix thoroughly. Add 1.0g of CaCO 3 to each of
50.0mL screw-capped bottles. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 25°C. Fill each bottle to capacity with enrichment me-
dium.
Use: For the cultivation of methanogenic bacteria.