Aero Pseudo Selective Agar

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Aero Pseudo Selective Agar
Composition per liter:
Agar ............................................................................................12.0g
Starch, soluble.............................................................................20.0g
Sodium glutamate.........................................................................2.0g
KH 2 PO 4 .........................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.5g
Phenol Red..................................................................................0.36g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Pimaricin.....................................................................................0.01g
Penicillin G....................................................................100,000 units
Preparation of Selective Supplement Solution:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add selective supplement solution.
Mix thoroughly. Pour into Petri dishes or aseptically distribute into
sterile tubes.
Use: For the selective cultivation of Pseudomonas spp. and Aeromo-
nas spp. For the detection of Aeromonas and Pseudomonas in foods,
water, and food processing equipment.