Methanococcus vannielii Medium

[所属分类:培养基配方] [发布时间:2021-5-24] [发布人:网站管理员2] [阅读次数:] [返回]

Methanococcus vannielii Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sodium formate ..........................................................................15.0g
K 2 HPO 4 .......................................................................................3.48g
CoCl 2 ·6H 2 O................................................................................2.38g
NH 4 Cl ...........................................................................................1.0g
L -Cysteine·HCl·H 2 O.....................................................................0.3g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O ................................................................................0.01g
FeSO 4 ·7H 2 O................................................................................0.01g
MnSO 4 ·H 2 O...............................................................................7.5mg
Na 2 MoO 4 ·2H 2 O .........................................................................7.5mg
Na 2 SeO 3 .....................................................................................1.7mg
Na 2 S·9H 2 O solution.................................................................10.0mL
Na 2 S·9H 2 O Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O..................................................................................0.15g
Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Prepare and distribute medium anaerobi-
cally under 100% N 2 . Add components, except Na 2 S·9H 2 O solution, to
distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Continue boiling until resazurin
turns colorless, indicating reduction. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 25°C. Aseptically add 10.0mL of sterile
Na 2 S·9H 2 O solution. Mix thoroughly. Aseptically and anaerobically
distribute into sterile tubes or flasks.
Use: For the cultivation of Methanococcus vannielii.