Methanobacterium espanolae Medium

[所属分类:培养基配方] [发布时间:2021-5-21] [发布人:网站管理员2] [阅读次数:] [返回]

Methanobacterium espanolae Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1020.0mL:

Sodium acetate·3H 2 O....................................................................2.5g
Na 2 CO 3 .........................................................................................0.5g
(NH 4 ) 2 SO 4 ...................................................................................0.45g
K 2 HPO 4 .......................................................................................0.29g
KH 2 PO 4 .......................................................................................0.18g
MgSO 4 ·7H 2 O..............................................................................0.12g
CaCl 2 ·2H 2 O.................................................................................0.06g
NaCl............................................................................................0.05g
L -Cysteine·HCl............................................................................0.25g
Na 2 S·9H 2 O..................................................................................0.25g
Resazurin ...................................................................................1.0mg
Trace elements solution ...........................................................10.0mL
Vitamin solution.......................................................................10.0mL
pH 5.5 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
MgSO 4 ·7H 2 O............................................................................... 3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O............................................................................... 0.5g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CaCl 2 ·2H 2 O...................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
CuSO 4 ·5H 2 O...............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with
KOH. Add remaining components. Add distilled/deionized water to
1.0L.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl........................................................................10.0mg
Calcium  DL -pantothenate...........................................................5.0mg
Lipoic acid .................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Riboflavin ..................................................................................5.0mg
Thiamine·HCl ............................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Adjust pH to 7.0. Mix thor-
oughly. Sparge with 80% N 2 + 20% CO 2 .
Preparation of Medium: Prepare and dispense the medium anaer-
obically with 80% N 2 and 20% CO 2 . Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to
5.5–6.0. Anaerobically distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–121°C. Check the pH of the medium after auto-
claving.
Use: For the cultivation of Methanobacterium espanolae.