Methanobacterium alcaliphilum Medium

[所属分类:培养基配方] [发布时间:2021-5-21] [发布人:网站管理员2] [阅读次数:] [返回]

Methanobacterium alcaliphilum Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaHCO 3 ......................................................................................10.0g
Yeast extract..................................................................................2.0g
Peptone..........................................................................................2.0g
NH 4 Cl ...........................................................................................1.0g
L- Cysteine·HCl·H 2 O......................................................................0.5g
K 2 HPO 4 .........................................................................................0.4g
MgCl 2 ·6H 2 O..................................................................................0.1g
CaCl 2 ...........................................................................................0.02g
Resazurin ...................................................................................1.0mg
Salt solution ...............................................................................5.0mL
pH 8.4 ± 0.2 at 25°C
Salt Solution:
Composition per 100.0mL:
Sodium EDTA·2H 2 O ....................................................................0.1g
CoCl 2 ·6H 2 O ................................................................................0.03g
MnCl 2 ·4H 2 O................................................................................0.02g
ZnCl 2 ...........................................................................................0.02g
AlCl 3 ·6H 2 O................................................................................8.0mg
CuCl 2 ·2H 2 O...............................................................................4.0mg
NiSO 4 ·6H 2 O...............................................................................4.0mg
Na 2 SeO 3 .....................................................................................2.7mg
FeSO 4 ·7H 2 O...............................................................................2.0mg
H 3 BO 3 ........................................................................................2.0mg
NaMoO 4 ·2H 2 O...........................................................................2.0mg
Preparation of Salt Solution: Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components, except NaHCO 3 , yeast
extract, peptone, and  L- cysteine·HCl·H 2 O, to distilled/deionized water
and bring volume to 990.0mL. Gently heat and bring to boiling under
O 2 -free 100% N 2 . Continue boiling until resazurin becomes pale, indi-
cating partial reduction. Add the yeast extract, peptone, and  L-
cysteine·HCl·H 2 O and continue boiling under O 2 -free 100% N 2 until
resazurin becomes colorless, indicating complete reduction. Cool to
room temperature under O 2 -free 100% N 2 . Add NaHCO 3 to 10.0mL of
distilled/deionized water. Mix thoroughly. Gas with O 2 -free 100% N 2
in a sealed tube. Add reduced NaHCO 3 solution to cooled reduced me-
dium. Distribute anaerobically into tubes. Cap with butyl rubber stop-
pers and secure with closures. Autoclave for 15 min at 15 psi pressure–
121°C with fast exhaust.
Use:  For the cultivation and maintenance of Methanobacterium alca-
liphilum.