Methanobacillus Medium

[所属分类:培养基配方] [发布时间:2021-5-21] [发布人:网站管理员2] [阅读次数:] [返回]

Methanobacillus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

KH 2 PO 4 .........................................................................................9.0g
K 2 HPO 4 .........................................................................................6.0g
NH 4 Cl ...........................................................................................5.0g
MgCl 2 ............................................................................................1.0g
CaCl 2 ...........................................................................................0.01g
FeSO 4 ·7H 2 O................................................................................0.01g
Ethanol.....................................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Filter sterilize ethanol. Add components,
except ethanol, to tap water and bring volume to 990.0mL. Mix thor-
oughly. Gently heat until dissolved. Autoclave for 20 min at 10psi pres-
sure–115°C. Cool to 45°–50°C. Aseptically add sterile ethanol. Mix
thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the selective isolation and cultivation of Methanobacillus
species from mixed cultures.