Metallogenium Medium

[所属分类:培养基配方] [发布时间:2021-5-21] [发布人:网站管理员2] [阅读次数:] [返回]

Metallogenium Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

MnCO 3 ..........................................................................................2.0g
Starch, hydrolyzed........................................................................1.0g
DNA............................................................................................0.01g
Catalase......................................................................................5.0mg
Mycoplasma broth base .........................................................100.0mL
Yeast extract, ultrafiltrate.......................................................100.0mL
Horse serum.............................................................................10.0mL
Mycoplasma Broth Base:
Composition per liter:
Pancreatic digest of casein............................................................7.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Beef heart, solids from infusion....................................................2.0g
Preparation of Mycoplasma Broth Base:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
MnCO 3 :
Composition per 100.0mL:
MnCl 2 ..........................................................................................20.0g
NaHCO 3 (25% solution)..........................................................25.0mL
Preparation of MnCO 3 : Add MnCl 2 to distilled/deionized water
and bring volume to 100.0mL. Mix thoroughly. Add NaHCO 3 solution.
Filter through Whatman #1 filter paper. Save the MnCO 3 precipitate.
Wash and store under distilled/deionized water.
Preparation of Medium:  Add MnCO 3 , hydrolyzed starch, and
DNA to 25.0mL of distilled/deionized water. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti-
cally add 100.0mL of sterile Mycoplasma broth base, 100.0mL of
ultrafiltrate of yeast extract, 10.0mL of horse serum, and 5.0mg of cat-
alase. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Metallogenium species.