Metallogenium Cultivation Broth

[所属分类:培养基配方] [发布时间:2021-5-21] [发布人:网站管理员2] [阅读次数:] [返回]

Metallogenium Cultivation Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Starch, hydrolyzed ......................................................................20.0g
MnCO 3 ..........................................................................................0.5g
MnCO 3 :
Composition per 100.0mL:
MnCl 2 ..........................................................................................20.0g
NaHCO 3 (25% solution)..........................................................25.0mL
Preparation of MnCO 3 : Add MnCl 2 to distilled/deionized water
and bring volume to 100.0mL. Mix thoroughly. Add NaHCO 3 solution.
Filter through Whatman #1 filter paper. Save the MnCO 3 precipitate.
Wash and store under distilled/deionized water.
Preparation of Medium: Hydrolyze starch with HCl. Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C.
Use: For the cultivation of Metallogenium species.