Activated Carbon Medium (DSMZ Medium 811)

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Activated Carbon Medium
(DSMZ Medium 811)
Composition per liter:
Agar............................................................................................15.0g
Na 2 HPO 4 ·12H 2 O...........................................................................9.0g
Activated carbon...........................................................................5.0g
KH 2 PO 4 .........................................................................................1.5g
NH 4 Cl ...........................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O .............................................................................20.0mg
NH 4 -Fe-III-Citrate.....................................................................1.2mg
Trace elements solution TS2......................................................1.0mL
pH 7.5 ± 0.1 at 25°C
Trace Elements Solution TS2:
Composition per liter:
Na 2 MoO 4 ·4H 2 O.....................................................................900.0mg
H 3 BO 3 ....................................................................................300.0mg
CoCl 2 ·6H 2 O...........................................................................200.0mg
ZnSO 4 ·7H 2 O..........................................................................100.0mg
MnCl 2 ·4H 2 O ............................................................................30.0mg
NiCl 2 ·6H 2 O..............................................................................20.0mg
Na 2 SeO 3 ...................................................................................20.0mg
CuCl 2 ·2H 2 O.............................................................................10.0mg
Trace Elements Solution TS2: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except activated carbon,
to distilled/deionized water and bring volume to 900.0mL. Mix thor-
oughly. After all components are dissolved add activated carbon. Bring
volume to 1.0L with distilled/deionized water. Adjust pH to 7.5. Distrib-
ute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Pour into Petri dishes or leave in tubes.
Use:  For the cultivation of Streptomyces thermoautotrophicus.