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培养基配方
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MeReSa Agar Base with Oxacillin (Methicillin-Resistant Staphylococcus aureus Agar)
[所属分类:培养基配方] [发布时间:2021-5-21] [发布人:网站管理员2] [阅读次数:] [返回]
MeReSa Agar Base with Oxacillin
(Methicillin-Resistant Staphylococcus aureus Agar)
Casein enzymic hydrolysate .......................................................10.0g
Glycine........................................................................................10.0g
Mannitol......................................................................................10.0g
NaCl............................................................................................10.0g
Sodium pyruvate.........................................................................10.0g
LiCl...............................................................................................5.0g
Beef extract...................................................................................5.0g
Indicator mix...............................................................................0.13g
MRSA selective supplement....................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without MRSA supplement solution, is avail-
able as a premixed powder from HiMedia.
Caution: Lithium chloride is harmful. Avoid bodily contact and inha-
lation of vapors. On contact with skin wash with plenty of water imme-
diately.
MRSA Selective Supplement:
Composition per 10.0mL:
Oxacillin ....................................................................................2.0mg
Preparation of MRSA Selective Supplement: Add oxacillin to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except MRSA selec-
tive supplement, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°C. Aseptically add 10.0mL MRSA selective supplement.
Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation and cultivation of methicillin-resistant Staphy-
lococcus aureus (MRSA).
(Methicillin-Resistant Staphylococcus aureus Agar)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar ............................................................................................20.0gCasein enzymic hydrolysate .......................................................10.0g
Glycine........................................................................................10.0g
Mannitol......................................................................................10.0g
NaCl............................................................................................10.0g
Sodium pyruvate.........................................................................10.0g
LiCl...............................................................................................5.0g
Beef extract...................................................................................5.0g
Indicator mix...............................................................................0.13g
MRSA selective supplement....................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without MRSA supplement solution, is avail-
able as a premixed powder from HiMedia.
Caution: Lithium chloride is harmful. Avoid bodily contact and inha-
lation of vapors. On contact with skin wash with plenty of water imme-
diately.
MRSA Selective Supplement:
Composition per 10.0mL:
Oxacillin ....................................................................................2.0mg
Preparation of MRSA Selective Supplement: Add oxacillin to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except MRSA selec-
tive supplement, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°C. Aseptically add 10.0mL MRSA selective supplement.
Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation and cultivation of methicillin-resistant Staphy-
lococcus aureus (MRSA).