Meniscus glaucopis Broth

[所属分类:培养基配方] [发布时间:2021-5-21] [发布人:网站管理员2] [阅读次数:] [返回]

Meniscus glaucopis Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Maltose .........................................................................................5.0g
Agar..............................................................................................3.0g
Yeast extract..................................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
NaCl..............................................................................................0.4g
NH 4 Cl ...........................................................................................0.4g
Sodium thioglycolate....................................................................0.3g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·H 2 O ..................................................................................0.01g
FeSO 4 ·7H 2 O...............................................................................1.0mg
Resazurin (0.025% solution) .....................................................4.0mL
Trace elements solution SL-6....................................................1.0mL
Vitamin solution.......................................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O ...............................................................................0.03g
Na 2 MoO 4 ·H 2 O ............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 · 2H 2 O.................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Vitamin Solution:
Composition per liter:
Vitamin B 12 ................................................................................2.8mg
Thiamine·HCl ..........................................................................0.28mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except vitamin solution,
to distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Adjust pH to 7.3 with 10% Na 2 CO 3 . Gently heat and bring to boiling.
Continue boiling until resazurin changes color. Cool to 50°C. Distribute
into tubes in 7.0mL volumes under O 2 -free 97% N 2 + 3% H 2 . Cap with
rubber stoppers under O 2 -free 97% N 2 + 3% H 2 . Place tubes in a press.
Autoclave for 15 min at 15 psi pressure–121°C using fast exhaust. Cool
to 50°C. Aseptically add 0.25mL of sterile vitamin solution to each tube.
Use: For the cultivation and maintenance of Meniscus glaucopis.