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M-Endo HiVeg Broth MF (MF Endo HiVeg Medium) (M-Coliform HiVeg Broth)
[所属分类:培养基配方] [发布时间:2021-5-21] [发布人:网站管理员2] [阅读次数:] [返回]
M-Endo HiVeg Broth MF
(MF Endo HiVeg Medium)
(M-Coliform HiVeg Broth)
Plant hydrolysate No. 1...............................................................10.0g
Plant hydrolysate...........................................................................5.0g
Plant special peptone ....................................................................5.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .....................................................................................4.375g
Na 2 SO 3 .........................................................................................2.1g
Yeast extract..................................................................................1.5g
KH 2 PO 4 .....................................................................................1.375g
Basic Fuchsin..............................................................................1.05g
Synthetic detergent No. III............................................................0.1g
Sodium lauryl sulfate..................................................................0.05g
pH 7.2 ± 0.1 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Caution: Basic Fuchsin is a potential carcinogen and care must be
taken to avoid inhalation of the powdered dye and contact with the
skin.
Preparation of Medium: Add ethanol to approximately 900.0mL
of distilled/deionized water. Add remaining components. Bring vol-
ume to 1.0L with distilled/deionized water. Mix thoroughly. Gently
heat and bring to boiling. Rapidly cool broth below 45°C. Do not auto-
clave. Use 1.8–2.0mL for each filter pad. Protect from the light. Pre-
pare broth freshly.
Use: For the cultivation and enumeration of coliform bacteria from
water by the membrane filter method.
(MF Endo HiVeg Medium)
(M-Coliform HiVeg Broth)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Lactose........................................................................................12.5gPlant hydrolysate No. 1...............................................................10.0g
Plant hydrolysate...........................................................................5.0g
Plant special peptone ....................................................................5.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .....................................................................................4.375g
Na 2 SO 3 .........................................................................................2.1g
Yeast extract..................................................................................1.5g
KH 2 PO 4 .....................................................................................1.375g
Basic Fuchsin..............................................................................1.05g
Synthetic detergent No. III............................................................0.1g
Sodium lauryl sulfate..................................................................0.05g
pH 7.2 ± 0.1 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Caution: Basic Fuchsin is a potential carcinogen and care must be
taken to avoid inhalation of the powdered dye and contact with the
skin.
Preparation of Medium: Add ethanol to approximately 900.0mL
of distilled/deionized water. Add remaining components. Bring vol-
ume to 1.0L with distilled/deionized water. Mix thoroughly. Gently
heat and bring to boiling. Rapidly cool broth below 45°C. Do not auto-
clave. Use 1.8–2.0mL for each filter pad. Protect from the light. Pre-
pare broth freshly.
Use: For the cultivation and enumeration of coliform bacteria from
water by the membrane filter method.
