M-Endo HiVeg Agar LES

[所属分类:培养基配方] [发布时间:2021-5-21] [发布人:网站管理员2] [阅读次数:] [返回]

M-Endo HiVeg Agar LES

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Lactose..........................................................................................9.4g
Plant hydrolysate No. 1.................................................................7.5g
NaCl..............................................................................................3.7g
Plant hydrolysate ..........................................................................3.7g
Plant peptone ................................................................................3.7g
K 2 HPO 4 .........................................................................................3.3g
Na 2 SO 3 .........................................................................................1.6g
Yeast extract..................................................................................1.2g
KH 2 PO 4 .........................................................................................1.0g
Basic Fuchsin................................................................................0.8g
Synthetic detergent No. III............................................................0.1g
Sodium lauryl sulfate..................................................................0.05g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Caution: Basic Fuchsin is a potential carcinogen and care must be
taken to avoid inhalation of the powdered dye and contact with the
skin.
Preparation of Medium:  Add ethanol to approximately 900.0mL
of distilled/deionized water. Add remaining components. Bring vol-
ume to 1.0L with distilled/deionized water. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile 60mm Petri dishes in 4.0mL volumes. Protect
from the light.
Use: For the cultivation and enumeration of coliform bacteria by the
membrane filter method.