Melissococcus plutonius Agar (LMG Medium 110)

[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]

Melissococcus plutonius Agar
(LMG Medium 110)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
Glucose.......................................................................................10.0g
Neopeptone...................................................................................5.0g
Peptone .........................................................................................2.5g
Yeast extract..................................................................................2.5g
Starch, soluble...............................................................................2.0g
Trypticase™..................................................................................2.0g
L -Cysteine·HCl ...........................................................................0.25g
Phosphate buffer solution........................................................50.0mL
Phosphate Buffer Solution:
Composition per liter:
KH 2 PO 4 .........................................................................................4.5g
Na 2 HPO 4 ·2H 2 O.............................................................................5.8g
Preparation of Phosphate Buffer Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 6.7.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. An-
aerobically distribute into tubes sparged with a gas mixture of 100% N 2
+ 10% CO 2 . Immediately plug with butyl rubber stoppers. Autoclave for
15 min at 15 psi pressure–121°C.
Use: For the cultivation of Melissococcus plutonius.