Melissococcus pluton Medium

[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]

Melissococcus pluton Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................10.0g
Neopeptone...................................................................................5.0g
Peptone..........................................................................................2.5g
Yeast extract..................................................................................2.5g
Soluble starch................................................................................2.0g
Pancreatic digest of casein............................................................2.0g
L- Cysteine·HCl·H 2 O....................................................................0.25g
Phosphate buffer (1M, pH 6.7) ................................................50.0mL
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2.
Gently heat and bring to boiling. Distribute into tubes or flasks that
have been flushed with 90% N 2 + 10% CO 2 . Cap with butyl rubber
stoppers. Place tubes in a press. Autoclave for 15 min at 15 psi pres-
sure–121°C.
Use:  For the cultivation and maintenance of Melissococcus pluton.