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培养基配方
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Medium for Hydrocarbon-Degrading Bacteria (Naphthalene Mineral Salts Medium)
[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]
Medium for Hydrocarbon-Degrading Bacteria
(Naphthalene Mineral Salts Medium)
(NH 4 ) 2 SO 4 .....................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.3g
CaCl 2 .............................................................................................0.1g
FeSO 4 ·7H 2 O................................................................................0.02g
Naphthalene...............................................................................2.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except naphthalene, to
distilled/deionized water and bring volume to 998.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 2.0mL of sterile
naphthalene to 20.0mL of sterile basal salts. Ultrasonically homoge-
nize the solution. Add the naphthalene–basal salts homogenate back to
the remainder of the sterile basal salts medium. Mix thoroughly. Asep-
tically distribute into sterile tubes or flasks.
Use: For the cultivation and enrichment of hydrocarbon-degrading
bacteria.
(Naphthalene Mineral Salts Medium)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
K 2 HPO 4 .........................................................................................1.0g(NH 4 ) 2 SO 4 .....................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.3g
CaCl 2 .............................................................................................0.1g
FeSO 4 ·7H 2 O................................................................................0.02g
Naphthalene...............................................................................2.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except naphthalene, to
distilled/deionized water and bring volume to 998.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 2.0mL of sterile
naphthalene to 20.0mL of sterile basal salts. Ultrasonically homoge-
nize the solution. Add the naphthalene–basal salts homogenate back to
the remainder of the sterile basal salts medium. Mix thoroughly. Asep-
tically distribute into sterile tubes or flasks.
Use: For the cultivation and enrichment of hydrocarbon-degrading
bacteria.
