Actinomyces Broth (DSMZ Medium 1029)

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Actinomyces Broth
(DSMZ Medium 1029)
Composition per liter:
Pancreatic digest of casein .........................................................17.0g
KH 2 PO 4 .......................................................................................15.0g
Yeast extract................................................................................10.0g
Glucose .........................................................................................5.0g
NaCl..............................................................................................5.0g
Heart muscle, solids from infusion...............................................2.0g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
L- Cysteine·HCl·H 2 O......................................................................1.0g
Soluble starch................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.01g
pH 6.9 ± 0.2 at 25°C
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the maintenance or cultivation of a variety of Actinomyces
species and other anaerobic bacteria.