Medium A

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

Medium A

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

D- Glucose....................................................................................20.0g
Agar............................................................................................20.0g
Yeast extract................................................................................10.0g
Biotin.........................................................................................1.0mg
Calcium pantothenate ................................................................1.0mg
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, except biotin and cal-
cium pantothenate, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Add biotin and
calcium pantothenate to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize. Aseptically add the sterile bi-
otin and calcium pantothenate solution to the cooled sterile basal me-
dium. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use:  For the cultivation and maintenance of Zymomonas mobilis.