MCA

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

MCA

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Carrots.......................................................................................200.0g
Agar ............................................................................................15.0g
Preparation of Medium: Peel and slice fresh carrots. Place carrots
in a blender. Add 500.0mL of distilled/deionized water. Blend for 40
sec at high speed. Filter through four layers of cheesecloth. Squeeze
out juice from the residue. Bring volume of filtrate to 1.0L with dis-
tilled/deionized water. Add agar. Mix thoroughly. Gently heat and
bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in
tubes.
Use:  For the cultivation of Phytophthora megasperma.