MBM Acetate Medium (Mineral Base Medium with Acetate)

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

MBM Acetate Medium
(Mineral Base Medium with Acetate)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................16.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................1.0g
NH 4 H 2 PO 4 ....................................................................................1.0g
Sodium acetate·3H 2 O....................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.1g
Bromthymol Blue .......................................................................0.01g
pH 6.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5. Gently
heat and bring to boiling. Distribute into screw-capped tubes in 3.0mL
volumes. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to
cool in a slanted position.
Use: For determining the nutritional independence of bacteria. Bacte-
ria that are nutritionally independent turn the medium blue.