M-Azide HiVeg Broth Base with Triphenyltetrazolium Chloride

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

M-Azide HiVeg Broth Base
with Triphenyltetrazolium Chloride

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Saccharose ................................................................................100.0g
Plant hydrolysate No. 1...............................................................40.0g
Yeast extract................................................................................10.0g
K 2 HPO 4 .........................................................................................4.0g
Glucose.........................................................................................2.0g
NaN 3 .............................................................................................0.4g
Triphenyltetrazolium chloride solution .....................................5.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Triphenyltetrazolium Choride Solution:
Composition per 5.0mL:
Triphenyltetrazolium chloride ......................................................0.1g
Preparation of Triphenyltetrazolium Choride Solution: Add
triphenyltetrazolium chloride to distilled/deionized water and bring
volume to 5.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except triphenyltetrazo-
lium chloride solution, to distilled/deionized water and bring volume to
1.0L.Mix thoroughly. Gently heat and bring to boiling. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 5.0mL triphenyltetrazolium chloride so-
lution. Mix thoroughly. Aseptically distribute into tubes.
Use: For the detection and enrichment of fecal streptococci in water
and sewage by the membrane filtration method.