Marine Spirochete Medium

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Spirochete Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Cellobiose .....................................................................................2.0g
Peptone..........................................................................................2.0g
Yeast extract..................................................................................1.0g
Sodium thioglycolate....................................................................1.0g
Seawater, charcoal filtered.....................................................800.0mL
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Add components, except sodium thiogly-
colate, to glass-distilled water and bring volume to 1.0L. Mix thor-
oughly. Bubble 100% N 2 into medium for 1.5 min. Add sodium
thioglycolate. Adjust pH to 7.5 with 10N KOH. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Spirochaeta bajacaliforn-
iensis.