Marine Pseudomonas Medium

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Pseudomonas Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Nutrient broth................................................................................8.0g
Yeast extract..................................................................................5.0g
Salt solution..................................................................................1.0L
Salt Solution:
Composition per liter:
NaCl..........................................................................................12.86g
MgCl 2 ..........................................................................................2.48g
KCl..............................................................................................0.75g
CaCl 2 ...........................................................................................0.56g
Fe(SO 4 ) 2 (NH 4 ) 2 ........................................................................0.048g
Preparation of Salt Solution: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to 1.0L of salt solution.
Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into
sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Alteromonas haloplank-
tis.