Marine Methanol Medium

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Methanol Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................20.0g
(NH 4 ) 2 SO 4 .....................................................................................2.0g
K 2 HPO 4 .........................................................................................2.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.3g
Methanol..................................................................................10.0mL
Vitamin B 12 solution................................................................10.0mL
Trace metals solution.................................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Vitamin B 12 Solution:
Composition per 100.0mL:
Vitamin B 12 ...............................................................................10.0μg
Preparation of Vitamin B 12 Solution: Add the vitamin B 12  to dis-
tilled/deionized water and bring volume to 100.0mL. Adjust pH to 5.

Autoclave for 15 min at 15 psi pressure–121°C.

Trace Metals Solution:
Composition per liter:
ZnSO 4 ·7H 2 O.................................................................................1.4g
MnSO 4 ·H 2 O................................................................................0.84g
FeSO 4 ·7H 2 O................................................................................0.28g
CuSO 4 ·5H 2 O...............................................................................0.25g
Na 2 MoO 4 ·2H 2 O..........................................................................0.24g
CoCl 2 ·6H 2 O................................................................................0.24g
CaCl 2 ·2H 2 O ................................................................................0.15g
Preparation of Trace Metals Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except vitamin B 12 so-
lution and methanol, to distilled/deionized water and bring volume to
980.0mL. Adjust pH to 7.0 with NaOH. Autoclave for 15 min at 15 psi
pressure–121°C. Filter sterilize methanol. Aseptically add sterile vita-
min B 12 solution and filter-sterilized methanol. Distribute into sterile
tubes or flasks.
Use:  For the cultivation and maintenance of Methylophaga thalassica.