Marine Glucose Trypticase ™ Yeast Extract Broth (MGTY Broth)

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Glucose Trypticase ™  Yeast Extract Broth
(MGTY Broth)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .........................................................................................2.0g
Pancreatic digest of casein............................................................1.0g
Yeast extract..................................................................................1.0g
L- Cysteine·HCl·H 2 O......................................................................0.5g
Seawater.................................................................................750.0mL
Tris-HCl buffer (5.0 mM, pH 7.5) ...........................................50.0mL
Resazurin (0.1% solution)..........................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Distribute into tubes or flasks under 97%
N 2 + 3% H 2 . Cap with rubber stoppers and place tubes in a press. Au-
toclave for 15 min at 15 psi pressure–121°C with fast exhaust.
Use:  For the cultivation and maintenance of Spirochaeta isovalerica.