Marine Flagellate Medium with B-Vitamins

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Flagellate Medium with B-Vitamins

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Seawater.................................................................................990.0mL
Vitamin solution.......................................................................10.0mL
Vitamin Solution:
Composition per 100.0mL:
Thiamine·HCl.............................................................................0.15g
Calcium  D -(+)-pantothenate .......................................................0.05g
Nicotinamide...............................................................................0.05g
Pyridoxal·HCl.............................................................................0.05g
Riboflavin ...................................................................................0.05g
Folic acid...................................................................................0.025g
Pyridoxamine·HCl ....................................................................0.025g
Biotin .......................................................................................12.5mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Allow natural seawater to age for 2
months. Filter sterilize. Aseptically add 100.0mL of sterile vitamin so-
lution. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation of Oikomonas species.