Marine Desulfovibrio Medium

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Desulfovibrio Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................980.0mL
Solution B................................................................................10.0mL
Solution C................................................................................10.0mL

pH 7.8 ± 0.2 at 25°C

Solution A:
Composition per 980.0mL:
NaCl............................................................................................25.0g
DL -Sodium lactate.........................................................................2.0g
MgSO 4 ·7H 2 O................................................................................2.0g
Na 2 SO 4 ..........................................................................................1.0g
NH 4 Cl ...........................................................................................1.0g
Yeast extract..................................................................................1.0g
K 2 HPO 4 .........................................................................................0.5g
CaCl 2 ·2H 2 O ..................................................................................0.1g
Resazurin...................................................................................1.0mg
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 980.0mL. Mix thoroughly. Gently heat and
bring to boiling. Continue boiling for 3–4 min. Allow to cool to room
temperature while gassing under 100% N 2 .
Solution B:
Composition per 10.0mL:
FeSO 4 ·7H 2 O..................................................................................0.5g
Preparation of Solution B:  Add FeSO 4 ·7H 2 O to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly.
Solution C:
Composition per 10.0mL:
Ascorbic acid................................................................................0.1g
Sodium thioglycolate....................................................................0.1g
Preparation of Solution C:  Add components to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Medium: To 980.0mL of cooled solution A, anaer-
obically add 10.0mL of solution B and 10.0mL of solution C. Mix thor-
oughly. Adjust pH to 7.8 with NaOH. Distribute into tubes or flasks.
During distribution, swirl the medium to keep the precipitate in suspen-
sion. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Desulfovibrio desulfuri-
cans, Desulfovibrio salexigens, and Desulfovibrio vulgaris.