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Marine Cytophaga Medium
[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]
Marine Cytophaga Medium
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
NaCl............................................................................................24.7g
Agar............................................................................................15.0g
MgSO 4 ·7H 2 O................................................................................6.3g
MgCl 2 ·6H 2 O .................................................................................4.6g
Tryptic digest of casein.................................................................1.0g
Yeast extract..................................................................................1.0g
KCl................................................................................................0.7g
NaHCO 3 solution.....................................................................10.0mL
CaCl 2 ·2H 2 O solution................................................................10.0mL
pH 7.2 ± 0.2 at 25°C
NaHCO 3 Solution:
Composition per 10.0mL:
NaHCO 3 ........................................................................................0.2g
Preparation of NaHCO 3 Solution: Add NaHCO3 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
CaCl 2 ·2H 2 O Solution:
Composition per 10.0mL:
CaCl 2 ·2H 2 O ..................................................................................1.2g
Preparation of CaCl 2 ·2H 2 O Solution: Add CaCl 2 ·2H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except NaHCO 3 solu-
tion and CaCl 2 ·2H 2 O solution, to distilled/deionized water and bring
volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 10.0mL of sterile NaHCO 3 solution and 10.0mL of
sterile CaCl 2 ·2H 2 O solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation of Cytophaga species, Flexibacter species,
Microscilla species, and Saprospira grandis.
NaCl............................................................................................24.7g
Agar............................................................................................15.0g
MgSO 4 ·7H 2 O................................................................................6.3g
MgCl 2 ·6H 2 O .................................................................................4.6g
Tryptic digest of casein.................................................................1.0g
Yeast extract..................................................................................1.0g
KCl................................................................................................0.7g
NaHCO 3 solution.....................................................................10.0mL
CaCl 2 ·2H 2 O solution................................................................10.0mL
pH 7.2 ± 0.2 at 25°C
NaHCO 3 Solution:
Composition per 10.0mL:
NaHCO 3 ........................................................................................0.2g
Preparation of NaHCO 3 Solution: Add NaHCO3 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
CaCl 2 ·2H 2 O Solution:
Composition per 10.0mL:
CaCl 2 ·2H 2 O ..................................................................................1.2g
Preparation of CaCl 2 ·2H 2 O Solution: Add CaCl 2 ·2H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except NaHCO 3 solu-
tion and CaCl 2 ·2H 2 O solution, to distilled/deionized water and bring
volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 10.0mL of sterile NaHCO 3 solution and 10.0mL of
sterile CaCl 2 ·2H 2 O solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation of Cytophaga species, Flexibacter species,
Microscilla species, and Saprospira grandis.
