Marine Chlorobiaceae Medium 2

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Chlorobiaceae Medium 2
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Solution 1...............................................................................950.0mL
Na 2 S·9H 2 O solution.................................................................60.0mL
NaHCO 3 solution.....................................................................40.0mL
Vitamin B 12 solution..................................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Solution 1:
Composition per 950.0mL:
NaCl............................................................................................20.0g
MgSO 4 ·7H 2 O................................................................................3.0g
KH 2 PO 4 .........................................................................................1.0g
NH 4 Cl ...........................................................................................0.5g
CaCl 2 ·2H 2 O ................................................................................0.05g
Trace elements solution SL-8....................................................1.0mL
Preparation of Solution 1:  Add components to distilled/deionized
water and bring volume to 950.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Trace Elements Solution SL-8:
Composition per liter:
Disodium EDTA...........................................................................5.2g
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O................................................................................0.19g
MnCl 2 ·4H 2 O .................................................................................0.1g
ZnCl 2 ...........................................................................................0.07g
H 3 BO 3 .........................................................................................0.06g
NaMoO 4 ·2H 2 O............................................................................0.04g
CuCl 2 ·2H 2 O................................................................................0.02g
NiCl 2 ·6H 2 O.................................................................................0.02g
Preparation of Trace Elements Solution SL-8: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly.
Na 2 S·9H 2 O Solution:
Composition per 100.0mL:
Na 2 S·9H 2 O....................................................................................5.0g
Preparation of Na 2 S·9H 2 O Solution:  Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 100.0mL. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
NaHCO 3 Solution:
Composition per 100.0mL:
NaHCO 3 ........................................................................................5.0g
Preparation of NaHCO 3 Solution:  Add NaHCO 3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Vitamin B 12 Solution:
Composition per 100.0mL:
Vitamin B 12 ................................................................................2.0mg
Preparation of Vitamin B 12 Solution:  Add vitamin B 12 to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  To 950.0mL of cooled, sterile solution 1,
aseptically add 60.0mL of sterile Na 2 S·9H 2 O solution, 40.0mL of ster-
ile NaHCO 3 solution, and 1.0mL of sterile vitamin B 12 solution. Mix
thoroughly. Adjust pH to 6.8 with sterile H 2 SO 4 or Na 2 CO 3  . Aseptical-
ly distribute into sterile 50.0mL or 100.0mL bottles with metal screw
caps and rubber seals. Completely fill bottles with medium except for

a pea-sized air bubble.

Use: For the isolation and cultivation of marine members of the Chlo-
robiaceae.