Marine Broth with Naphthalene

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Broth with Naphthalene

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..........................................................................................19.45g
MgCl 2 ............................................................................................8.8g
Peptone .........................................................................................5.0g
Na 2 SO 3 ........................................................................................3.24g
CaCl 2 .............................................................................................1.8g
Yeast extract..................................................................................1.0g
KCl..............................................................................................0.55g
NaHCO 3 ......................................................................................0.16g
Ferric citrate..................................................................................0.1g
KBr .............................................................................................0.08g
SrCl 2 ............................................................................................0.03g
H 3 BO 3 .........................................................................................0.02g
Na 2 HPO 4 ....................................................................................8.0mg
Na 2 SiO 3 ......................................................................................4.0mg
NaF ............................................................................................2.4mg
NH 4 NO 3 .....................................................................................1.6mg
Naphthalene..................................................................................1mg

pH 7.6 ± 0.2 at 25°C

Preparation of Medium: Add components, except naphthalene, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add a few crytals of naphthalene to each tube or
flask.
Use: For the cultivation of naphthalene-utilizing marine bacteria.