Marine Ameba Medium

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Ameba Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................10.0g
Malt extract...................................................................................0.1g
Yeast extract..................................................................................0.1g
Artificial seawater.........................................................................1.0L
Artificial Seawater:
Composition per liter:
NaCl............................................................................................27.5g
MgSO 4 ·7H 2 O..............................................................................6.78g
MgCl 2 ·6H 2 O ...............................................................................5.38g
KCl..............................................................................................0.72g
NaHCO 3 ....................................................................................... 0.2g
CaCL 2 ·2H 2 O.................................................................................1.4g
Preparation of Artificial Seawater: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to artificial seawater
and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation of Cochliopodium clarum, Heteramoeba
clara, Lingulamoeba leei, Paramoeba pemaquidensis, and Vannella
species.