Marine Agar with Naphthalene

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Agar with Naphthalene

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..........................................................................................19.45g
Agar............................................................................................15.0g
MgCl 2 ............................................................................................8.8g
Peptone..........................................................................................5.0g
Na 2 SO 3 ........................................................................................3.24g
CaCl 2 .............................................................................................1.8g
Yeast extract..................................................................................1.0g
KCl..............................................................................................0.55g
NaHCO 3 ......................................................................................0.16g
Ferric citrate..................................................................................0.1g
KBr..............................................................................................0.08g
SrCl 2 ............................................................................................0.03g
H 3 BO 3 .........................................................................................0.02g
Na 2 HPO 4 ....................................................................................8.0mg
Na 2 SiO 3 ......................................................................................4.0mg
NaF.............................................................................................2.4mg
NH 4 NO 3 .....................................................................................1.6mg
Naphthalene..................................................................................1mg
pH 7.6 ± 0.2 at 25°C
Preparation of Medium: Add components, except naphthalene, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes. After agar solidifies, aseptically add a few crystals of
naphthalene to each plate.
Use: For the cultivation and maintenance of naphthalene-utilizing
marine bacteria