Malonate Broth, Ewing Modified

[所属分类:培养基配方] [发布时间:2021-5-12] [发布人:网站管理员2] [阅读次数:] [返回]

Malonate Broth, Ewing Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sodium malonate ..........................................................................3.0g
NaCl..............................................................................................2.0g
(NH 4 ) 2 SO 4 .....................................................................................2.0g
Yeast extract..................................................................................1.0g
Glucose .......................................................................................0.25g
K 2 HPO 4 .........................................................................................0.6g
KH 2 PO 4 .........................................................................................0.4g
Bromthymol Blue .....................................................................0.025g
pH 6.7 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and differentiation of coliforms and other
enteric organisms, particularly Enterobacter and Escherichia, based on
their ability to utilize malonate as a carbon source and ammonium sul-
fate as a nitrogen source. The small amount of yeast extract and glu-
cose encourages the growth of some organisms that may be distressed
or fail to respond. Malonate-utilizing organisms turn the medium blue.