Maleate Medium for Pseudomonas fluorescens

[所属分类:培养基配方] [发布时间:2021-5-12] [发布人:网站管理员2] [阅读次数:] [返回]

Maleate Medium for Pseudomonas fluorescens

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
K 2 HPO 4 .......................................................................................1.13g
NH 4 NO 3 ........................................................................................1.0g
KH 2 PO 4 .......................................................................................0.48g
MgSO 4 ·7H 2 O................................................................................0.2g
Potassium maleate solution......................................................8.61mL
pH 7.0 ± 0.2 at 25°C
Potassium Maleate Solution:
Composition per liter:
Maleic acid.............................................................................. 116.07g
KOH (10N solution) ..............................................................200.0mL
Preparation of Potassium Maleate Solution:  Add maleic acid
to distilled/deionized water and bring volume to 600.0mL. Slowly add
KOH solution (generates heat). Bring volume to 1.0L with distilled/de-
ionized water. Adjust pH to 7.0. Filter sterilize.
Preparation of Medium:  Add components, except potassium
maleate solution, to distilled/deionized water and bring volume to
991.4mL. Mix thoroughly. Gently heat while stirring until boiling. Au-
toclave for 20 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically
add 8.61mL of the potassium maleate solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Pseudomonas fluorescens
and Mycoplasma pneumoniae.