MacConkey Broth

[所属分类:培养基配方] [发布时间:2021-5-11] [发布人:网站管理员2] [阅读次数:] [返回]

MacConkey Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of gelatin.........................................................20.0g
Lactose........................................................................................10.0g
Oxgall ...........................................................................................5.0g
Bromcresol Purple......................................................................0.02g
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. If testing 10.0mL samples, prepare
medium double strength. Mix thoroughly. Gently heat while stirring
until boiling. Distribute into test tubes containing inverted Durham
tubes. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the selective isolation and cultivation of coliforms in milk
and water.