MacConkey Agar, Fluorocult (Fluorocult MacConkey Agar)

[所属分类:培养基配方] [发布时间:2021-5-11] [发布人:网站管理员2] [阅读次数:] [返回]

MacConkey Agar, Fluorocult
(Fluorocult MacConkey Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone from casein....................................................................17.0g
Agar ............................................................................................13.5g
Lactose........................................................................................10.0g
NaCl..............................................................................................5.0g
Peptone from meat........................................................................3.0g
Bile salt mixture............................................................................1.5g
4-Methylumbelliferyl-β- D -glucuronide........................................0.1g
Neutral Red.................................................................................0.03g
Crystal Violet............................................................................0.001g
pH 7.1 ± 0.2 at 25°C
Source: This medium is available from Merck.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Pour into sterile Petri
dishes. The plates are clear and red to red-brown.
Use:  For the isolation of Salmonella, Shigella, and coliform bacteria,
in particular E. coli, from various materials. The bile salts and Crystal
Violet largely inhibit the growth of Gram-positive microbial flora. Lac-
tose together with the pH indicator Neutral Red are used to detect lac-
tose-positive colonies and E. coli can be seen among these because of
fluorescence under UV light.