MacConkey Agar

[所属分类:培养基配方] [发布时间:2021-5-11] [发布人:网站管理员2] [阅读次数:] [返回]

MacConkey Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of gelatin.........................................................17.0g
Agar............................................................................................13.5g
Lactose........................................................................................10.0g
NaCl..............................................................................................5.0g
Bile salts........................................................................................1.5g
Pancreatic digest of casein............................................................1.5g
Peptic digest of animal tissue .......................................................1.5g
Neutral Red.................................................................................0.03g
Crystal Violet.............................................................................1.0mg
pH 7.1 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring until boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the selective isolation, cultivation, and differentiation of coli-
forms and enteric pathogens based on the ability to ferment lactose.
Lactose-fermenting organisms appear as red to pink colonies. Lactose-
nonfermenting organisms appear as colorless or transparent colonies.