Acidianus infernus Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidianus infernus Medium
Composition per liter:
(NH 4 ) 2 SO 4 .....................................................................................1.3g
Sulfur flowers ...............................................................................1.0g
KH 2 PO 4 .......................................................................................0.28g
MgSO 4 ·7H 2 O..............................................................................0.25g
CaCl 2 ·2H 2 O.................................................................................0.07g
FeCl 3 ·6H 2 O.................................................................................0.02g
Yeast extract................................................................................0.02g
Na 2 B 4 O 7 ·10H 2 O.........................................................................4.5mg
MnCl 2 ·4H 2 O...............................................................................1.8mg
ZnSO 4 ·7H 2 O............................................................................0.22mg
CuCl 2 ·2H 2 O .............................................................................0.05mg
Na 2 MoO 4 ·2H 2 O .......................................................................0.03mg
VOSO 4 ·2H 2 O...........................................................................0.03mg
CoSO 4 ......................................................................................0.01mg
pH 2.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 2.5 with
10N H 2 SO 4 . Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use: For the aerobic cultivation and maintenance of Desulfurolobus
ambivalens, Acidianus brierleyi, and Acidianus infernus.