M9 Medium with Tryptophan (DSMZ Medium 451)

[所属分类:培养基配方] [发布时间:2021-5-11] [发布人:网站管理员2] [阅读次数:] [返回]

M9 Medium with Tryptophan
(DSMZ Medium 451)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

10X M9 salts..........................................................................100.0mL
Glucose solution......................................................................10.0mL
Calcium chloride solution........................................................10.0mL
Magnesium sulfate solution.....................................................10.0mL
Thiamine solution......................................................................1.0mL
Proline solution..........................................................................1.0mL
Tryptophan solution...................................................................1.0mL
pH 7.4 ± 0.2 at 25°C
Glucose Solution:
Composition per 10.0mL:
D- Glucose......................................................................................2.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Thiamine Solution:
Composition per 10.0mL:
Thiamine - HCl·2H 2 O ...................................................................3.7g
Preparation of Thiamine Solution: Add thiamine - HCl·2H 2 O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Tryptophan Solution:
Composition per 10.0mL:
Tryptophan....................................................................................0.2g
Preparation of Tryptophan Solution: Add tryptophan to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Proline Solution:
Composition per 10.0mL:
Proline...........................................................................................0.2g
Preparation of Proline Solution: Add proline to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Calcium Chloride Solution:
Composition per 10.0mL:
CaCl 2 ............................................................................................0.1g
Preparation of Calcium Chloride Solution: Add CaCl 2 to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Magnesium Sulfate Solution:
Composition per 10.0mL:
MgSO 4 ..........................................................................................1.2g
Preparation of Magnesium Sulfate Solution: Add MgSO 4 to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
10X M9 Salts Solution:
Composition per liter:
Na 2 HPO 4 .....................................................................................60.0g
KH 2 PO 4 .......................................................................................30.0g
NH 4 Cl .........................................................................................10.0g
NaCl..............................................................................................5.0g
MgSO 4 ..........................................................................................1.2g
Preparation of 10X M9 Salts Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Aseptically combine sterile component
solutions. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use:  For the cultivation of Escherichia coli tryptophan auxotrophs.