M9 Medium with Casamino Acids

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

M9 Medium with Casamino Acids

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 .......................................................................................6.0g
Casamino acids.............................................................................5.0g
KH 2 PO 4 .........................................................................................3.0g
NH 4 Cl ...........................................................................................1.0g
NaCl..............................................................................................0.5g
Glucose solution ......................................................................10.0mL
MgSO 4 ·7H 2 O solution...............................................................1.0mL
Thiamine·HCl solution ..............................................................1.0mL
CaCl 2 solution............................................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Glucose solution:
Composition per 100.0mL:
D- Glucose....................................................................................20.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
MgSO 4 ·7H 2 O Solution:
Composition per liter:
MgSO 4 ·7H 2 O............................................................................246.5g
Preparation of MgSO 4 ·7H 2 O Solution: Add MgSO 4 ·7H 2 O to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Thiamine·HCl Solution:
Composition per 10.0mL:
Thiamine·HCl ..........................................................................10.0mg
Preparation of Thiamine·HCl Solution: Add thiamine·HCl to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
CaCl 2 Solution:
Composition per liter:
CaCl 2 solution.............................................................................14.7g
Preparation of CaCl 2 Solution: Add CaCl 2 solution to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except MgSO 4 ·7H 2 O
solution, glucose solution, thiamine·HCl solution, and CaCl 2 solution,
to distilled/deionized water and bring volume to 987.0mL. Mix thor-
oughly. Adjust pH to 7.0. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to room temperature. Aseptically add sterile
MgSO 4 ·7H 2 O solution, sterile glucose solution, sterile thiamine·HCl
solution, and sterile CaCl 2 solution. Mix thoroughly. Distribute into
tubes or flasks.
Use:  For the cultivation and maintenance of Flavobacterium menin-
gosepticum.