M9 Medium with Arginine

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

M9 Medium with Arginine

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1013.0mL:

L -Proline ..................................................................................20.0mg
L -Arginine................................................................................20.0mg
10X M9 salts..........................................................................100.0mL
Glucose solution......................................................................10.0mL
CaCl 2 ·2H 2 O solution..................................................................1.0mL
MgSO 4  solution..........................................................................1.0mL
Thiamine·HCl solution ..............................................................1.0mL
pH 7.4 ± 0.2 at 25°C
10 X M9 Salts:
Composition per liter:
Na 2 HPO 4 .....................................................................................60.0g
KH 2 PO 4 .......................................................................................30.0g
NH 4 Cl .........................................................................................10.0g
NaCl..............................................................................................5.0g
Preparation of 10 X M9 Salts : Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4.
Glucose Solution:
Composition per 100.0mL:
Glucose.......................................................................................20.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
CaCl 2 ·2H 2 O Solution:
Composition per 100.0mL:
CaCl 2 ·2H 2 O ................................................................................1.47g
Preparation of CaCl 2 ·2H 2 O Solution:  Add CaCl 2 ·2H 2 O to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
MgSO 4  Solution:
Composition per 100.0mL:
MgSO 4 ......................................................................................12.04g
Preparation of MgSO 4  Solution:  Add MgSO 4 to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Thiamine·HCl Solution:
Composition per 10.0mL:
Thiamine·HCl .............................................................................3.37g
Preparation of Thiamine·HCl Solution:  Add thiamine·HCl to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add  L -proline,  L -arginine, and 10X M9
salts to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically
add 10.0mL of sterile glucose solution, 1.0mL of sterile CaCl 2 ·2H 2 O
solution, 1.0mL of sterile MgSO 4  solution, and 1.0mL of sterile
thiamine·HCl solution. Mix thoroughly. Aseptically distribute into
sterile tubes or flasks.
Use: For the cultivation of Escherichia coli.